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RSTREP-RO

Roche

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

Synonym(s):

streptavidin

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About This Item

UNSPSC Code:
12352202

biological source

Streptomyces sp. ( Streptomyces avidinii)

Quality Level

recombinant

expressed in E. coli

form

liquid

specific activity

≥17 units/mg protein

mol wt

~52 kDa

packaging

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

manufacturer/tradename

Roche

concentration

70-90%

technique(s)

protein array: suitable
protein purification: suitable

impurities

6-7% water

pH range

5.0-9.0

pI 

7.4-7.7 (two main bands)

solubility

water: 10 mg/L (mA405=100)

suitability

suitable for immunological tests

UniProt accession no.

shipped in

cooler

storage temp.

-10 to -25°C

Related Categories

General description

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Sequence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Unit Definition

One unit binds 1 μg D-biotin at pH 8.9.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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