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Carbol-Fuchsin solution according to Kinyoun

for microscopy

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Synonym(s):
Kinyoun’s carbol-Fuchsin
MDL number:
PubChem Substance ID:

grade

for microscopy

Quality Level

form

liquid

composition

fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)

impurities

phenol

color

red to very dark red

density

0.99 g/mL at 20 °C

ε (extinction coefficient)

77 at 546 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]

InChI

1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3

InChI key

IMOKRVRFBCWWGH-UHFFFAOYSA-N

Related Categories

General description

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

Application

  • Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
  • It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
  • It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

Principle

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

Other Notes

Stain used for the demonstration of tuberculosis bacteria

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

109.9 °F - closed cup

flash_point_c

43.3 °C - closed cup


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Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

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