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33152

Supelco

1,5-Diphenylcarbazide

reag. Ph. Eur., ≥98.0%, for metal titration

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Synonym(s):
1,5-Diphenylcarbohydrazide, sym.-Diphenylcarbazide
Linear Formula:
C6H5NHNHCONHNHC6H5
CAS Number:
Molecular Weight:
242.28
Beilstein/REAXYS Number:
752039
EC Number:
MDL number:
PubChem Substance ID:

grade

for metal titration

Quality Level

agency

USP/NF
reag. Ph. Eur.

assay

≥98% (HPLC)
≥98.0%

technique(s)

UV/Vis spectroscopy: suitable
titration: suitable

impurities

diphenylcarbazone, in accordance
≤0.1% insoluble matter in ethanol

ign. residue

≤0.05% (as SO4)

mp

170-175 °C (lit.)
170-175 °C

suitability

passes test for Hg detection

SMILES string

O=C(NNc1ccccc1)NNc2ccccc2

InChI

1S/C13H14N4O/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14-15H,(H2,16,17,18)

InChI key

KSPIHGBHKVISFI-UHFFFAOYSA-N

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This Item
D7766259225T7394
1,5-Diphenylcarbazide reag. Ph. Eur., ≥98.0%, for metal titration

33152

1,5-Diphenylcarbazide

1,5-Diphenylcarbazide reagent grade

D7766

1,5-Diphenylcarbazide

1,5-Diphenylcarbazide ACS reagent

259225

1,5-Diphenylcarbazide

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

170-175 °C (lit.)

mp

170-175 °C (lit.)

mp

170-175 °C (lit.), 173-176 °C

mp

49-51 °C (lit.)

grade

for metal titration

grade

reagent grade

grade

ACS reagent

grade

-

agency

USP/NF

agency

-

agency

-

agency

-

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

1,5-Diphenylcarbazide reaction with chromium(VI), has been widely used for the spectro- photometric determination of chromium. It is also an electron donor, and can be used for measuring photosystem 2 activity in subchloroplast fragments.

Application

1,5-diphenylcarbazide was used for preparing diphenylcarbazide solution, during an experimental procedure done for reducing biological chromium(VI), using trickling filter. It may be used for pre-column complexation in HPLC, during on-line preconcentration and determination of chromium(VI) traces in water.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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On-line preconcentration and determination of chromium (VI) in waters by high-performance liquid chromatography using pre-column complexation with 1, 5-diphenylcarbazide.
Padarauskas A
Journal of Chromatography A, 808 (1), 193-199 (1998)
Oxidation of 1, 5-diphenylcarbazide as a measure of photosystem 2 activity in subchloroplast fragments.
Vernon LP and Shaw ER.
Biochemical and Biophysical Research Communications, 36 (6), 878-884 (1969)
Biological chromium (VI) reduction using a trickling filter.
Dermou E
Journal of Hazardous Materials, 126 (1), 78-85 (2005)
N Rajesh et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 69(2), 612-618 (2007-07-03)
A novel approach has been developed for the solid phase extraction of chromium(VI) based on the adsorption of its diphenylcarbazide complex on a mixture of acid activated montmorillonite (AAM)-silica gel column. The effect of various parameters such as acidity, stability
A Padarauskas et al.
Journal of chromatography. A, 808(1-2), 193-199 (1998-07-04)
A method for on-line chromatographic preconcentration and determination of chromium(VI) traces has been developed. Chromate was preconcentrated on a C18 column (50 x 6 mm I.D.) after complexation with diphenylcarbazide (DPC). Following the preconcentration step, analysis of the sample was

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