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Millipore

RLS Broth

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
Rapid Lemonade Spoilage Organism Broth, Rapid Lemonade Spoiling Organism Broth, SSL Broth
UNSPSC Code:
41171607

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

D(+)-Biotin (Vitamin H), 100 μg/L
Fructose, 10 g/L
Glucose, 30 g/L
Malt Extract, 200 g/L
Orange Extract (=Orangenserum 10 °Brix (60 °Brix) 400g/l (60 g/l)), 40 g/L
Saccharose, 30 g/L
Yeast Extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

5.0±0.2 (25 °C)

application(s)

bioburden testing
food and beverages
sample preparation

microbiology

suitability

Acetobacter spp.

Related Categories

Application

Rapid enrichment medium for the beverage spoiling microorganisms. It can be used for all kind of beverage, base ingredients or swap samples in the soft drink, dairy and winery industry.

Preparation Note

Dissolve 313 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes or sterile filter the solution. For normal beverages mix add 50 ml broth to 200 mL of sample. For base ingredients 10-20 mL of sample are added to 50 mL broth. For filtered samples the rolled membrane is given in a sterile tube or flask with broth. It is recommended to degas carbonated beverages before performing analysis. Incubate under aerobic conditions for 2 days at a temperature of 25-29°C.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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