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B3801

Millipore

Vitamin B12 assay medium

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Vitamin B12 Assay Medium, Cyanocobalamin Assay Medium

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About This Item

UNSPSC Code:
41171608
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

packaging

bottle of 100 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

light amber

final pH

6.1±0.2 (25 °C)

application(s)

agriculture
food and beverages
pharmaceutical

microbiology

storage temp.

2-8°C

suitability

nonselective for Lactobacillus spp.

Related Categories

General description

Vitamin B12 Assay Medium is a Vitamin B12 free medium containing all other vitamins and nutrients essential for the growth of Lactobacillus leichmannii ATCC 7830 as the test organism. Cyanocobalamin (Vitamin B12, Cat.No. 95190 ) is added in increasing concentrations giving a growth response that can be measured turbidimetrically or acidimetrically. A standard curve is plotted with absorbance as a function of the vitamin B12 concentration. The concentration of vitamin B12 in the test sample is calculated based on the interpretation of the standard curve. Lactobacillus species grow poorly on non-selective culture media and require special nutrients for their growth. Vitamin assay media are prepared for use in the microbiological assay of vitamins. Three types of media used for the microbiological assay of vitamins are:

  • The maintenance media used for carrying the stock culture
  • The inoculum media for preparation of the inoculum
  • The assay media for quantitation of the vitamin under test.

Application

Vitamin B12 Assay Medium is a Vitamin B12 free medium recommended for microbiological assay of Vitamin B12 from various test samples

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Components

Ingredients (g/L)
Casein acid hydrolysate, 15.00
Dextrose, 40.00
Asparagine, 0.20
Sodium acetate, 20.00
Ascorbic acid, 4.00
L-Cystine, 0.40
DL-Tryptophan, 0.40
Adenine sulfate, 0.02
Guanine hydrochloride, 0.02
Uracil, 0.02
Xanthine, 0.02
Riboflavin (Vitamin B2), 0.001
Thiamine hydrochloride, 0.001
Biotin, 0.00001
Niacin, 0.002
p-Amino benzoic acid, 0.002
Calcium pantothenate, 0.001
Pyridoxine hydrochloride, 0.004
Pyridoxal hydrochloride, 0.004
Pyridoxamine hydrochloride, 0.0008
Folic acid, 0.0002
Monopotassium phosphate, 1.00
Dipotassium phosphate, 1.00
Magnesium sulfate, 0.40
Sodium chloride, 0.02
Ferrous sulfate, 0.02
Manganese sulfate, 0.02
Polysorbate 80, 2.00

Preparation Note

Suspend 8.5 g of Vitamin B12 Assay Medium in 100 ml of distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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The United States Pharmacopeia XX
United States Pharmacopeia (1990)

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