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C6852

Sigma-Aldrich

L-Cysteine hydrochloride monohydrate

from non-animal source, suitable for cell culture, meets EP, USP testing specifications

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Synonym(s):
L-Cysteine hydrochloride hydrate (1:1:1)
Linear Formula:
HSCH2CH(NH2)COOH · HCl · H2O
CAS Number:
Molecular Weight:
175.63
Beilstein/REAXYS Number:
5158059
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

biological source

non-animal source

Quality Level

agency

meets EP testing specifications
meets USP testing specifications

assay

98.5-101.0% dry basis

form

powder or crystals

optical activity

[α]20/D 6.1 to 7.8°, c = 8 in 1 M HCl
[α]25/D 5.7 to 6.8°, c = 8 in HCl (6 N)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

solubility

water: 1 g/10 mL, clear, colorless

cation traces

Fe: ≤10 ppm
NH4+: ≤0.02%

application(s)

pharmaceutical (small molecule)

functional group

(sulfhydryl)

storage temp.

room temp

SMILES string

O.Cl.N[C@@H](CS)C(O)=O

InChI

1S/C3H7NO2S.ClH.H2O/c4-2(1-7)3(5)6;;/h2,7H,1,4H2,(H,5,6);1H;1H2/t2-;;/m0../s1

InChI key

QIJRTFXNRTXDIP-JIZZDEOASA-N

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This Item
PHR110277845130122
functional group

(sulfhydryl)

functional group

-

functional group

-

functional group

-

assay

98.5-101.0% dry basis

assay

-

assay

98.5-101.0%

assay

98.5-101.0%

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

storage temp.

room temp

storage temp.

2-30°C

storage temp.

-

storage temp.

-

color

white

color

-

color

-

color

-

General description

L-Cysteine hydrochloride, commonly referred to as Cysteine HCl, is a vital component in biochemical research due to its versatile roles. Derived from the naturally occurring amino acid L-cysteine, this compound plays a crucial part in redox reactions, protein structure, cell culture, and antioxidant activity. Cysteine HCl functions as a reducing agent and a nucleophile in redox reactions. Additionally, it serves as a precursor to glutathione, a potent cellular antioxidant. Its significance extends to protein structure, where it contributes to the formation of disulfide bonds that stabilize protein structures. Cysteine HCl also supports cell growth and viability in cell culture applications. Its widespread applications encompass diverse areas of biochemical research, including redox biology, protein biochemistry, cell biology, and antioxidant investigations.

Application

L-Cysteine hydrochloride monohydrate has been used as a component of yeast dropout media for culturing yeasts. It has also been used as a component of differential reinforced clostridial broth (DRCM) for the isolation of clostridia.

Biochem/physiol Actions

Cysteine (Cys) is a sulfur-containing amino acid that modulates metabolism for growth, reproduction and immunity. It provides the disulfide linkage in proteins and in involved in the transport of sulfur. Cysteine is involved in the formation of hydrogen sulfide that functions as a signaling molecule. Cys residues are present at the catalytic sites of various enzymes.
NMDA glutamatergic receptor antagonist.

Features and Benefits

  • Suitable for Cell Culture and Biochemical research
  • High-quality compound suitable for multiple research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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L-Cysteine hydrochloride United States Pharmacopeia (USP) Reference Standard

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