MilliporeSigma
All Photos(4)

Documents

A7094

Sigma-Aldrich

L-Asparagine monohydrate

from non-animal source, BioReagent, suitable for cell culture, ≥98.0%

Sign Into View Organizational & Contract Pricing

Synonym(s):
Asparagine monohydrate, (S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide
Linear Formula:
NH2COCH2CH(NH2)COOH · H2O
CAS Number:
Molecular Weight:
150.13
Beilstein:
1723527
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

biological source

non-animal source

Quality Level

product line

BioReagent

Assay

≥98.0%

form

powder

optical activity

[α]20/D 33.0 to 36.3 °, c = 10 in 6 M HCl

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

233-235 °C (lit.)

solubility

H2O: 20 mg/mL

cation traces

Fe: ≤10 ppm
heavy metals (as Pb): ≤10 ppm
<0.1% (Ammonia (NH3))

functional group

amide
amine
carboxyl

storage temp.

room temp

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A4284A8381RES10070-A7
L-Asparagine monohydrate from non-animal source, BioReagent, suitable for cell culture, &#8805;98.0%

Sigma-Aldrich

A7094

L-Asparagine monohydrate

Essential+ Grade
L-Asparagine monohydrate BioReagent, suitable for cell culture

Sigma-Aldrich

A4284

L-Asparagine monohydrate

-
L-Asparagine monohydrate &#8805;99% (TLC)

Sigma-Aldrich

A8381

L-Asparagine monohydrate

-
L-Asparagine monohydrate

SAFC

RES10070-A7

L-Asparagine monohydrate

-
assay

≥98.0%

assay

≥99% (TLC)

assay

≥99% (TLC)

assay

98.5-101.0% dry basis

form

powder

form

powder

form

powder

form

powder

optical activity

[α]20/D 33.0 to 36.3 °, c = 10 in 6 M HCl

optical activity

-

optical activity

-

optical activity

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

endotoxin and microbial, tested

General description

L-Asparagine is a naturally occurring amino acid found in various proteins. It is used in laboratory research for various applications, including cell culture, protein biochemistry, and nitrogen metabolism studies. Its solubility in water and role as a source of asparagine make it a versatile tool for investigating biological processes and chemical synthesis. Further, L-Asparagine residues in proteins can be crucial for protein stability, folding, and interactions.

Application

L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. L-Asparagine, a proteinogenic amino acid, from non-animal source is used in biomanufacturing cell culture systems for the production of therapeutic recombinant proteins and monoclonal antibodies. It has also been used as a component of LHC basal media for the culture of human airway epithelial cells.

Biochem/physiol Actions

The amino acid L-asparagine is a structural analog of L-aspartic acid, where the side chain of the carboxylic acid moiety is amidated to give a terminal amine group. This renders L-asparagine neutral at physiological pH. The amide group of asparagine is derived from glutamine, in the reaction of aspartate and glutamine in the presence of ATP to yield asparagine and glutamate. In vivo, asparagine is hydrolyzed to aspartate and NH4+ by asparaginase. Asparagine is also an important amino acid in glycopeptide bonds, via N-glycosyl linkages to the sugar rings.

Features and Benefits

  • Suitable for Cell Culture and Biochemical research
  • High-quality compound suitable for multiple research applications

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 10

1 of 10

L-Alanine from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Sigma-Aldrich

A7469

L-Alanine

L-Serine from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Sigma-Aldrich

S4311

L-Serine

L-Glutamic acid from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%

Sigma-Aldrich

G8415

L-Glutamic acid

Asparagine anhydrous United States Pharmacopeia (USP) Reference Standard

USP

1043502

Asparagine anhydrous

L-Proline from non-animal source, meets EP, USP testing specifications, suitable for cell culture

Sigma-Aldrich

P5607

L-Proline

L-Glutamine meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

Sigma-Aldrich

G8540

L-Glutamine

Asparagine monohydrate United States Pharmacopeia (USP) Reference Standard

USP

1043513

Asparagine monohydrate

L-Tyrosine from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 99.0-101.0%

Sigma-Aldrich

T8566

L-Tyrosine

Handbook of Chem-ical Neu-roanatomy (2014)
Methods in Molecular Medicine, 107 null
Stryer L
Biochemistry (2002)
T Berg et al.
Molecular genetics and metabolism, 73(1), 18-29 (2001-05-15)
Lysosomal alpha-mannosidase (EC 3.2.1.24) is required in the degradation of the asparagine-linked carbohydrates of glycoproteins. Deficiency of this enzyme leads to the lysosomal storage disorder alpha-mannosidosis. As an initial step toward enzyme replacement therapy for alpha-mannosidosis, the human lysosomal alpha-mannosidase
S R Heller et al.
Journal of biotechnology, 145(4), 334-340 (2010-01-14)
Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service