650498

Chloroform

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

• Synonym(s): Methylidyne trichloride, Trichloromethane
• Empirical Formula (Hill Notation): CHCl₃
• CAS Number: 67-66-3
• Molecular Weight: 119.38
• Beilstein/REAXYS Number: 1731042
• EC Number: 200-663-8
• MDL number: MFCD00000826
• UNSPSC Code: 12190000
• PubChem Substance ID: 329760024
• NACRES: NA.04
• grade: HPLC Plus
• assay: ≥99.9%
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• application(s): food and beverages
• bp: 60.5-61.5 °C (lit.)
• vapor pressure: 160 mmHg ( 20 °C)

Properties

• grade: HPLC Plus
• Quality Level: 100
• vapor density: 4.1 (vs air)
• vapor pressure: 160 mmHg ( 20 °C)
• assay: ≥99.9%
• form: liquid
• contains: amylenes as stabilizer
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• impurities: ≤0.02% water; ≤0.5 ppb Fluorescence (quinine) at 365 nm
• evapn. residue: ≤0.0001%

Description

General description

Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent.

Application

Chloroform may be used in the following:

• Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods
• Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD)
• Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabinol (THC) residues on hands of suspected consumers by ion-mobility spectrometry (IMS)
• Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification
• Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS
• Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry

Chloroform was used in the making of silanized coverslips for DNA combing.[1]
It may be used as:

• Extractant in solvent extraction process.[2]
• A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.[3]
• Solvent for recrystallization.[4]

Suitable for HPLC, spectrophotometry, environmental testing

Packaging

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Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302,H315,H319,H331,H336,H351,H361d,H372
• pcodes: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• target_organs: Central nervous system, Liver,Kidney
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: does not flash
• flash_point_c: does not flash