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Sigma-Aldrich

Fluorescein isothiocyanate isomer I

≥97.5% (HPLC)

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Synonym(s):
FITC, Fluorescein 5-isothiocyanate
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
Beilstein:
1407295
EC Number:
MDL number:
PubChem Substance ID:

Assay

≥97.5% (HPLC)

form

powder

impurities

≤1.5% Isomer II

mp

>360 °C (lit.)

solubility

acetone: soluble 0.1 g/10 mL

fluorescence

λex 492 nm; λem 518 nm (green)
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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This Item
F7250F365146935
form

powder

form

powder

form

powder

form

solid

mp

>360 °C (lit.)

mp

>360 °C (lit.)

mp

-

mp

-

solubility

acetone: soluble 0.1 g/10 mL

solubility

acetone: 1 mg/mL

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 492 nm; λem 518 nm (green), λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

fluorescence

λex 492 nm; λem 518 nm (green)

fluorescence

-

fluorescence

λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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