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49419

Sigma-Aldrich

L-Glutamine

BioUltra, ≥99.5% (NT)

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Synonym(s):
(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
Beilstein:
1723797
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26

product line

BioUltra

Quality Level

Assay

≥99.5% (NT)

form

powder

optical activity

[α]20/D +33.0±1°, c = 5% in 5 M HCl

technique(s)

cell culture | mammalian: suitable

impurities

insoluble matter, passes filter test
≤0.5% foreign amino acids

ign. residue (900 °C)

≤0.1% (as SO4)

loss

≤0.1% loss on drying, 110 °C

color

white

pH

5.0-6.0 (25 °C, 0.1 M in H2O)

mp

185 °C (dec.) (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

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This Item
G8540G6392G5792
L-Glutamine BioUltra, ≥99.5% (NT)

Sigma-Aldrich

49419

L-Glutamine

Premium Grade
L-Glutamine meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

Sigma-Aldrich

G8540

L-Glutamine

-
L-Glutamine γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

G6392

L-Glutamine

-
L-Glutamine

SAFC

G5792

L-Glutamine

-
assay

≥99.5% (NT)

assay

99.0-101.0%

assay

-

assay

≥99%

form

powder

form

powder

form

powder

form

powder

optical activity

[α]20/D +33.0±1°, c = 5% in 5 M HCl

optical activity

[α]20/D 31.5 to 33.0°, c = 10 in 2 M HCl, [α]20/D 6.3 to 7.3°, c = 4 in water

optical activity

-

optical activity

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

insoluble matter, passes filter test, ≤0.5% foreign amino acids

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, heavy metals, residual solvents, tested

Application

L-Glutamine has been used in culture medium for hypothalamic neurons, spacer in isotachophoresis of serum proteins, growth requirement of various microorganisms.

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T. Yagi et al. et al.
Electrophoresis ?83, 503-503 (1984)
Techniques for culture of hypothalamic neurons.
C Loudes et al.
Methods in enzymology, 103, 313-334 (1983-01-01)
CRC Handbook of Microbiology, 4, 1-1 (1974)
Rebekah M Samsonraj et al.
Stem cells (Dayton, Ohio), 33(6), 1878-1891 (2015-03-11)
This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and in vivo attributes of cells isolated from the bone marrow of age- and sex-matched donors. Adherence to plastic was not indicative of potency
Pablo Hernández-Varas et al.
Nature communications, 6, 7524-7524 (2015-06-26)
Cell-matrix adhesions are central mediators of mechanotransduction, yet the interplay between force and adhesion regulation remains unclear. Here we use live cell imaging to map time-dependent cross-correlations between vinculin-mediated tension and adhesion complex area, revealing a plastic, context-dependent relationship. Interestingly

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