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G2150

Sigma-Aldrich

L-Glutamine solution

200 mM, Hybri-Max, sterile-filtered, suitable for hybridoma

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Synonym(s):
L-Glutamine for cell culture
CAS Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75

grade

Hybri-Max

Quality Level

sterility

sterile-filtered

Assay

≥99% (TLC)

form

solution

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

color

clear colorless

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

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This Item
59202CG579276523
L-Glutamine solution 200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

Sigma-Aldrich

G2150

L-Glutamine solution

L-Glutamine Solution 200 mM 29.23 mg/mL in saline, solution, suitable for cell culture

SAFC

59202C

L-Glutamine Solution 200 mM

L-Glutamine

SAFC

G5792

L-Glutamine

L-Glutamine certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Supelco

76523

L-Glutamine

assay

≥99% (TLC)

assay

-

assay

≥99%

assay

-

form

solution

form

solution

form

powder

form

-

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, heavy metals, residual solvents, tested

impurities

-

color

clear colorless

color

-

color

-

color

white

General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Importance and uses of glutamine in hybridoma and mammalian cell culture

Chromatograms

application for HPLC

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