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81189

Sigma-Aldrich

Poly(ethylene glycol)

BioUltra, for molecular biology, 1,000

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Synonym(s):
PEG
Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

grade

for molecular biology

product line

BioUltra

form

solid

mol wt

Mr 950-1050

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5-7.0 (25 °C, 50 mg/mL in H2O)

solubility

H2O: 50 mg/mL at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

50 mg/mL in H2O

UV absorption

λ: 260 nm Amax: ≤0.10
λ: 280 nm Amax: ≤0.05

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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1 of 4

This Item
812538125581210
vibrant-m

81189

Poly(ethylene glycol)

Premium Grade
vibrant-m

81253

Poly(ethylene glycol)

Premium Grade
vibrant-m

81255

Poly(ethylene glycol)

Premium Grade
vibrant-m

81210

Poly(ethylene glycol)

-
Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

Quality Level

200

mol wt

Mr 950-1050

mol wt

Mr 5000-7000

mol wt

Mr 5000-7000

mol wt

average Mw 1,500

cation traces

Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Ca: ≤10 mg/kg, Co: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤200 mg/kg, Mg: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Sr: ≤5 mg/kg, As: ≤0.1 mg/kg, Cd: ≤5 mg/kg, Fe: ≤5 mg/kg, Mn: ≤5 mg/kg, Pb: ≤5 mg/kg, Bi: ≤5 mg/kg, Li: ≤5 mg/kg, Zn: ≤5 mg/kg, Cr: ≤5 mg/kg, Na: ≤200 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤200 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤200 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤200 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤200 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

cation traces

-

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

insoluble matter, passes filter test, ≤20 mg/kg peroxides (as H2O2)

impurities

-

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.

Other Notes

PEG precipitation of DNA fragments

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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J.T. Lis
Methods in Enzymology, 65, 86139-86139 (1980)
Positive and negative immunoselection for enrichment of two classes of osteoprogenitor cells.
Turksen K and Aubin JE
The Journal of Cell Biology, 114, 373-373 (1991)
High-frequency transformation of Brevibacterium lactofermentum protoplasts by plasmid DNA.
Santamaria RI, et al.
Journal of Bacteriology, 162, 463-463 (1985)
Poly(Ethylene Glycol) Chemistry: Biotechnical and Biomedical Applications (2013)
Poly (ethylene glycol) in drug delivery: pros and cons as well as potential alternatives.
Katrin K, et al.
Angewandte Chemie (International Edition in English), 49, 6288-6308 (2010)

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