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85639

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Synonym(s):

Saline sodium citrate buffer dry blend

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

product line

BioReagent

form

powder blend

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

solubility

H2O: 0.263 g/mL, clear, colorless

storage temp.

room temp

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Components

  • Convenient pack sizes
  • Multi-purpose use in hybridizations
  • Easy-to-use powder makes fast, inexpensive solutions
SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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