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85639

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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Synonym(s):
Saline sodium citrate buffer dry blend
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioReagent

form

powder blend

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

solubility

H2O: 0.263 g/mL, clear, colorless

storage temp.

room temp

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This Item
S0902T752711666681001
vibrant-m

85639

SSC Buffer

-
vibrant-m

S0902

SSC Buffer

-
vibrant-m

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
grade

for molecular biology

grade

-

grade

for molecular biology

grade

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-

form

powder blend

form

powder blend

form

powder blend

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

20-25°C

impurities

DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected

impurities

-

impurities

DNase and RNase, none detected

impurities

-

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Components

  • Convenient pack sizes
  • Multi-purpose use in hybridizations
  • Easy-to-use powder makes fast, inexpensive solutions
SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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