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Sigma-Aldrich

4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg trifluoroacetate salt

≥95% (HPLC)

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Synonym(s):
Pz-Pro-Leu-Gly-Pro-D-Arg trifluoroacetate salt
Empirical Formula (Hill Notation):
C38H52N10O8 · xC2HF3O2
Molecular Weight:
776.88 (free base basis)
UNSPSC Code:
12352204
NACRES:
NA.32

Quality Level

assay

≥95% (HPLC)

form

powder

Related Categories

Application

4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg trifluoroacetate salt has been used as substrate for collagenase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Thimet oligopeptidase (EC 3.4.24.15) is a thiol-dependent metallo-endopeptidase also known as Pz-peptidase, collagenase-like peptidase, endooligopeptidase A, soluble metallo-endopeptidase and endopeptidase 24.15. The enzyme is closely related to the yeast proteinase yscD. Thimet oligopeptidase (M(r) 74000) is widely distributed in animals
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Highly sensitive assay for PZ-peptidase activity by high-performance liquid chromatography
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A rapid and sensitive assay method for the determination of PZ-peptidase activity is reported. This method is based on the monitoring of the absorption at 320 nm of 4-phenylazobenzyloxycarbonyl-L-Pro-L-Leu (PZ-Pro-Leu), enzymatically formed from the substrate 4-phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg (PZ-peptide), after separation by

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