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90101

Sigma-Aldrich

Ampliflu Red

for fluorescence, ≥98.0% (HPLC)

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Synonym(s):
10-Acetyl-3,7-dihydroxyphenoxazine
Empirical Formula (Hill Notation):
C14H11NO4
CAS Number:
Molecular Weight:
257.24
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for fluorescence

Quality Level

assay

≥98.0% (HPLC)

form

powder

fluorescence

λex 571 nm; λem 585 nm in DMSO

storage temp.

2-8°C

SMILES string

CC(=O)N1c2ccc(O)cc2Oc3cc(O)ccc13

InChI

1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3

InChI key

PKYCWFICOKSIHZ-UHFFFAOYSA-N

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This Item
145338368987444
Ampliflu™ Red for fluorescence, ≥98.0% (HPLC)

90101

Ampliflu Red

Rhodamine B for fluorescence

83689

Rhodamine B

grade

for fluorescence

grade

for fluorescence

grade

for fluorescence

grade

for fluorescence

form

powder

form

-

form

powder or crystals

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

fluorescence

λex 571 nm; λem 585 nm in DMSO

fluorescence

λex 340 nm; λem 510 nm, λex 355 nm; λem 465 nm in TE buffer; DNA

fluorescence

λex 553 nm; λem 627 nm in methanol

fluorescence

λex 492 nm; λem 517 nm

Application

Ampliflu Red has been used to study mitochondrial abnormalities and oxidative stress related to Parkinson’s Disease. Ampliflu Red assay was suitable as an alternative indicator assay for detecting the inhibition of hTPO (human thyroid peroxidase) activity. Phospholipase D, an essential cellular process ingredient, used Ampliflu Red (10-Acetyl-3,7-dihydroxyphenoxazine) to determine its activity.Ampliflu Red is used for the following applications:
  • Quantification of inflammatory mediators
  • Determination of free and esterified cholesterol contents
  • Evaluation of compounds as substrates for GABA-AT compounds

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

related product

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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