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N2415

Sigma-Aldrich

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

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Synonym(s):
PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

recombinant

expressed in E. coli

Quality Level

assay

≥80% protein basis (biuret)

form

lyophilized powder

specific activity

≥10 units/mg protein

sequence note

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

UniProt accession no.

storage temp.

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

Gene Information

Escherichia coli ... deoD(945654)

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This Item
T7915P6635D9380
Nucleoside Phosphorylase bacterial recombinant, expressed in E. coli, ≥10 units/mg protein

N2415

Nucleoside Phosphorylase bacterial

Phosphorylase b from rabbit muscle lyophilized powder, ≥20 units/mg protein, 2× crystallization

P6635

Phosphorylase b from rabbit muscle

assay

≥80% protein basis (biuret)

assay

-

assay

-

assay

-

Gene Information

Escherichia coli ... deoD(945654)

Gene Information

Escherichia coli K12 ... trxB(949054)

Gene Information

-

Gene Information

Escherichia coli K12 ... polA(948356)

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

form

buffered aqueous glycerol solution

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

UniProt accession no.

P0ABP8

UniProt accession no.

P0A9P4

UniProt accession no.

A0A5F9CLZ9, A0A5F9CRR0, A0A5F9D1C4, A0A5F9DLL9

UniProt accession no.

P00582

Application

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Biochem/physiol Actions

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Unit Definition

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis

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