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I5907

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Pyrophosphatase, Inorganic from Escherichia coli

recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

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Synonym(s):
Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

assay

≥90%

form

lyophilized powder

specific activity

≥800 units/mg protein

mol wt

hexamer subunit mol wt 20 kDa

storage temp.

−20°C

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1 of 4

This Item
P5931I1643F3174
Fpg Protein from Escherichia coli ≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

F3174

Fpg Protein from Escherichia coli

assay

≥90%

assay

-

assay

-

assay

≥90% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

expressed in E. coli

form

lyophilized powder

form

lyophilized powder

form

powder

form

buffered aqueous glycerol solution

mol wt

hexamer subunit mol wt 20 kDa

mol wt

-

mol wt

71 kDa

mol wt

30.2 kDa (269 amino acids, predicted from the nucleotide sequence)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.
Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

Other Notes

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg2+ dependent. It is a relatively thermostable protein.

Unit Definition

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

Physical form

Lyophilized powder in Tris-buffered salts containing protease inhibitors

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Heterologous overexpression of genes in Escherichia coli has made it possible to obtain high titers of recombinant proteins. However, this can result in the formation of aggregated protein particles known as 'inclusion bodies'. Protein sequestered as inclusion body is inactive

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