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Y22001

Sigma-Aldrich

EnPresso® Y Defined

Growth system for expressing protein in yeast

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sterility

sterile; γ-irradiated

form

tablet

pH

6.8

shipped in

ambient

storage temp.

room temp

Biochem/physiol Actions

EnPresso® Y Defined is a pre-sterilized growth system designed to increase the yield of functional proteins expressed in Pichia pastoris when using AOX promoters. The system provides stress-free conditions, faster growth and eliminates the need for time-consuming media changes required when using conventional methanol-induction. EnPresso® growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling cultures to reach far higher cell densities than those achieved using conventional media.

By controlling growth rate and metabolism, a greater proportion of expressed protein can be correctly folded to improve solubility, minimize the risk of inclusion body formation, and ensure functionality of the final product.

EnPresso® growth systems maintain pH, provide adequate minerals, vitamins and trace elements to support growth, and use proprietary EnBase technology to ensure a constant, slow release of glucose from a polysaccharide substrate.

See all available products from EnPresso B Growth Systems.

Physical form

EnPresso® B is supplied in a kit providing sufficient reagents for 40 separate 50 ml cultures. Included in the kit:
120 tablets in 40 orange bags
1 bottle (20 ml) Reagent A

Legal Information

EnBase is a trademark of BioSilta Oy
EnPresso is a registered trademark of BioSilta Oy

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