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A1293

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Goat Anti-Mouse IgG (Fab specific)–AP
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A2179A1682A2429
biological source

goat

biological source

goat

biological source

goat

biological source

goat

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:40,000, western blot (chemiluminescent): 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25, western blot (chemiluminescent): 1:80,000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10, western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:160,000-1:320,000

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Specificity

Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.

Immunogen

Purified mouse IgG Fab fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:
  • enzyme-linked immunosorbent assay (ELISA){47
  • immunohistochemistry
  • western blot

Biochem/physiol Actions

Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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