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MilliporeSigma

A9207

Avidin−Agarose from egg white

aqueous glycerol suspension

Synonym(s):

Avidin agarose

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.56
MDL number:
MFCD00130572
Form:
aqueous glycerol suspension
Biological source:
egg white

Key Documents

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biological source

egg white

conjugate

agarose conjugate

form

aqueous glycerol suspension

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino

matrix spacer

15 atoms

storage temp.

−20°C

Quality Level

200

Related Categories

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
  • in avidin bead assay for purification of labelled biotin
  • for capture of glycoproteins from murine cornea samples

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Other Notes

One unit will bind 1.0 μg of d-biotin.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000507151
0000507163
0000507163
0000507151
0000512643

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Systematic evaluation of avidin-biotin interaction by fluorescence spectrophotometry
Sun, S., et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 89C, 99-104 (2011)
Amber L Jolly et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to
Dielectric spectroscopy of molecular interactions based on the avidin-biotin complex
Mellor, B.L., et al.
Conference proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 3660-3663 (2011)
Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione
Kato Y, et al.
Redox Biology, 2, 983-990 (2014)
Jens Spross et al.
Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)
A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric
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