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A9253

Sigma-Aldrich

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

Synonym(s):

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

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About This Item

CAS Number:
9000-89-9
Enzyme Commission number:
1.4.3.2 (BRENDA, IUBMB)
EC Number:
232-564-0
MDL number:
MFCD00131454
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Crotalus adamanteus

Quality Level

200

type

Type I (dried venom)
Type I

form

powder

mol wt

~130 kDa

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

storage temp.

−20°C

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General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Characterization of L-amino Acid Oxidase Derived from Crotalus adamanteus Venom: Procoagulant and Anticoagulant Activities
Nielsen VG
International Journal of Molecular Sciences, 20(19), 4853-4853 (2019)
An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process
Borges EP and Reis BF
Journal of the Brazilian Chemical Society, 16(6), 1226-1232 (2005)
The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
Rokyta DR, et al.
BMC Genomics, 13(1), 312-312 (2012)
Alba Martín-Barreiro et al.
Analytical and bioanalytical chemistry, 414(8), 2641-2649 (2022-01-23)
An enzymatic-colorimetric method has been developed based on the reaction between L-phenylalanine (L-Phe) and the L-amino acid oxidase (LAAO) in the presence of Au(III), which has led to the formation of gold nanoparticles. The intensity of the localized surface plasmon
Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro
Raibekas AA and Massey V
Proceedings of the National Academy of Sciences of the USA, 93(15), 7546-7551 (1996)
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