Skip to Content
MilliporeSigma
All Photos(1)

Documents

A9253

Sigma-Aldrich

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

Synonym(s):

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Crotalus adamanteus

Quality Level

type

Type I (dried venom)
Type I

form

powder

mol wt

~130 kDa

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

pictograms

Skull and crossbones

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Characterization of L-amino Acid Oxidase Derived from Crotalus adamanteus Venom: Procoagulant and Anticoagulant Activities
Nielsen VG
International Journal of Molecular Sciences, 20(19), 4853-4853 (2019)
The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
Rokyta DR, et al.
BMC Genomics, 13(1), 312-312 (2012)
Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro
Raibekas AA and Massey V
Proceedings of the National Academy of Sciences of the USA, 93(15), 7546-7551 (1996)
Alba Martín-Barreiro et al.
Analytical and bioanalytical chemistry, 414(8), 2641-2649 (2022-01-23)
An enzymatic-colorimetric method has been developed based on the reaction between L-phenylalanine (L-Phe) and the L-amino acid oxidase (LAAO) in the presence of Au(III), which has led to the formation of gold nanoparticles. The intensity of the localized surface plasmon
Linrui Wu et al.
Organic & biomolecular chemistry, 18(32), 6236-6240 (2020-07-31)
4-Fluorothreonine (4-FT) is the only naturally occurring fluorinated amino acid antibiotic. Although two conserved proteins in the 4-FT pathway have been found to be involved in self-detoxification mechanisms, the 4-FT-producing strains may also require an alternative pathway to degrade the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service