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Annexin V-Cy3 Apoptosis Detection Kit

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Synonym(s):
Red fluorescent Annexin V apoptosis assay
NACRES:
NA.32

usage

sufficient for 200 tests

Quality Level

packaging

pkg of 1 kit

technique(s)

flow cytometry: suitable

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

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This Item
APOAFTOX8TOX4
usage

sufficient for 200 tests

usage

(20 tests)

usage

 kit sufficient for 2,000 tests

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

detection method

fluorometric

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of Cytiva

Kit Components Only

Product No.
Description

  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit Components Also Available Separately

Product No.
Description
SDS

  • C50416-Carboxyfluorescein diacetate 10 mgSDS

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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