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B0519

Sigma-Aldrich

Biotin−Agarose

PBS suspension

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Synonym(s):
Vitamin H Agarose
MDL number:
NACRES:
NA.56

form

PBS suspension

Quality Level

extent of labeling

≥30 mg avidin per mL packed resin
2-6 μmol per mL

matrix

4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix spacer

13 atoms

application(s)

life science and biopharma

storage temp.

2-8°C

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This Item
B0301S1638B4501
Sigma-Aldrich

B0519

Biotin−Agarose

Biotin meets USP testing specifications

B0301

Biotin

Biotin ≥99% (HPLC), lyophilized powder

B4501

Biotin

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

extent of labeling

≥30 mg avidin per mL packed resin

extent of labeling

-

extent of labeling

≥1 mg per mL

extent of labeling

-

matrix

4% beaded agarose

matrix

-

matrix

4% beaded agarose

matrix

-

matrix activation

epoxy

matrix activation

-

matrix activation

cyanogen bromide

matrix activation

-

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix attachment

-

matrix attachment

amino

matrix attachment

-

Application

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physical form

Suspension in phosphate buffered saline containing 0.02% sodium azide

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)
Emrah Celik et al.
Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
Dietmar Eberbeck et al.
Journal of nanobiotechnology, 6, 4-4 (2008-03-13)
The binding reaction of the biomolecules streptavidin and anti-biotin antibody, both labelled by magnetic nanoparticles (MNP), to biotin coated on agarose beads, was quantified by magnetorelaxometry (MRX). Highly sensitive SQUID-based MRX revealed the immobilization of the MNP caused by the
K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always

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