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B8400

Sigma-Aldrich

Monoclonal Anti-Bovine IgG2 antibody produced in mouse

clone BG2-7, ascites fluid

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MDL number:
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BG2-7, monoclonal

contains

15 mM sodium azide

technique(s)

capture ELISA: suitable
dot blot: suitable
indirect ELISA: 1:1,000
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
R0761I9513I5385
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

The IgG class antibodies contain two subclasses, IgG1 and IgG2, which have antigenic differences in the Fc portion of the heavy chains. Monoclonal Anti-Bovine IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG2 antibody has been used in:
  • western blot
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot assay

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration
Hurley IP, et al.
Journal of Dairy Science, 87(3), 543-549 (2004)
Detection of Bovine IgG Isotypes in a PPA-ELISA for Johne's Disease Diagnosis in Infected Herds
Fernandez B, et al.
Veterinary Medicine International, 2012 (2012)
Macarena Sarli et al.
PloS one, 15(2), e0229301-e0229301 (2020-02-23)
Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via vaccination with live A. centrale produced in splenectomized calves. Since A.
Tom N McNeilly et al.
Vaccine, 28(5), 1412-1421 (2009-11-21)
Systemic immunization of cattle with H7 flagellin results in induction of both H7-specific IgA and IgG antibodies but only partially protects against subsequent colonization with Escherichia coli O157:H7. Recent studies indicate that anti-flagellin antibodies directed against TLR5 binding domains located

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