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C9972

Sigma-Aldrich

Cholera Toxin B subunit

biotin conjugate, lyophilized powder

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Synonym(s):
CTB
MDL number:
PubChem Substance ID:
NACRES:
NA.77

conjugate

biotin conjugate

Quality Level

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~40% Lowry

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

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1 of 4

This Item
C1655C167C3741
vibrant-m

C9972

Cholera Toxin B subunit

vibrant-m

C1655

Cholera Toxin B subunit

vibrant-m

C167

Cholera Toxin B subunit

vibrant-m

C3741

Cholera Toxin B subunit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mol wt

~12 kDa

mol wt

~12 kDa

mol wt

~12 kDa

mol wt

pentamer 57 kDa, ~12 kDa

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

peroxidase conjugate (Contains ~ 2 moles HRP/mole of CTB. ~100 μg HRP conjugated to ~45 μg CTB)

composition

Protein, ~40% Lowry

composition

Protein, ~20% Lowry

composition

-

composition

-

General description

Cholera Toxin B (CTB) is secreted by Vibrio cholerae. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea. It acts as a mucosal immunogen. Cholera toxin (CT) stimulates cell surface molecules, such as antigen presenting cells (APCs), murine and human dendritic cells (DCs). CT also has immunomodulatory properties. It induces the secretion of interleukin 1 (IL-1) from macrophages and enhances their APC function.

Application

Cholera Toxin B subunit has been used:
  • in immunofluorescence
  • in the analysis of major histocompatibility complex (MHC) class II lipid raft partitioning
  • in live cell three-dimensional tracking of SH-SY5Y human neuroblastoma cells
  • to assess the toll-like receptors (TLR) and FcRγ (Fc receptor γ chain) – CARD9 (caspase recruitment domain family member 9) activation by cholera Toxin B (CTB)

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Quality

Biotin content ~1.0 mole/mole protein.

Physical form

Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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