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SAE0069

Sigma-Aldrich

Cholera Toxin B subunit

recombinant, expressed in HEK 293 cells, subunit A free

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Synonym(s):
CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB
NACRES:
NA.32

recombinant

expressed in HEK 293 cells

Quality Level

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

quality

(The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.)

specific activity

(Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)

mol wt

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing) (Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.)
calculated mol wt 13 kDa
observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

solubility

H2O: soluble 11 mg/mL

UniProt accession no.

storage temp.

−20°C

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This Item
C1655C9972C8180
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing) (Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.), calculated mol wt 13 kDa, observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

mol wt

~12 kDa

mol wt

~12 kDa

mol wt

-

solubility

H2O: soluble 11 mg/mL

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

Application

Cholera Toxin B subunit has been used:
  • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
  • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Si-Yuan Song et al.
Frontiers in neuroanatomy, 14, 600555-600555 (2020-12-18)
This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus. The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting

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