MilliporeSigma
All Photos(1)

Documents

ZR-75-1

87012601

Sign Into View Organizational & Contract Pricing

biological source

human breast

growth mode

Adherent

karyotype

2n = 46, modal no. 72

morphology

Epithelial

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptors

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian breast carcinoma

Cell Line Description

Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.

Application

Hormone and tumourigenicity studies
ZR-75-1 has been used to determine the effects of cucurbitacin E (CuE) extracted from Ecballium elaterium A.Rich., Fam. Cucurbitaceae.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9.3
TPOX: 8
vWA: 16,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow very slowly, do not form a confluent monolayer and attach only lightly to the substrate. After subculture, it can take up to 7 days before cells re-attach. Detachment of cells can occur in PBS and trypsinisation can produce clumps of cells. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. When subculturing, ensure the trypsin is completely removed by centrifuging the cells and resuspending them in fresh medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service