A375 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88113005, human skin, Epithelial

• Synonym(s): A-375 Cells, A375-MEL Cells
• UNSPSC Code: 41106514

Properties

• biological source: human skin
• description: Human malignant melanoma
• packaging: tube of 5 μg 88113005-DNA-5UG; pkg of vial of cells 88113005-1VL
• growth mode: Adherent
• karyotype: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Biochem/physiol Actions

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Human malignant melanoma

Preparation Note

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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