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Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

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Synonym(s):
Cellular glutathione peroxidase, c-GPx
NACRES:
NA.32

Quality Level

usage

sufficient for 100 tests

shelf life

Kit is stable for 24 months when unopened.

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

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This Item
CS0260A7340354102
vibrant-m

CS0260

Glutathione Assay Kit

vibrant-m

354102

Glutathione Assay Kit

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

detection method

colorimetric

detection method

-

detection method

-

detection method

colorimetric

usage

sufficient for 100 tests

usage

sufficient for 700 assays

usage

-

usage

sufficient for 100 tests

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shelf life

Kit is stable for 24 months when unopened.

shelf life

-

shelf life

-

shelf life

-

General description

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Suitability

Suitable for the measureent of glutathione peroxidase in tissue extracts

Principle

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Other Notes

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Kit Components Only

Product No.
Description

  • tert-Butyl hydroperoxide 1 mL

related product

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

wgk_germany

WGK 3


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