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G9297

Sigma-Aldrich

Glutathione Reductase human

buffered aqueous solution, ≥10 units/mg protein, recombinant, expressed in E. coli

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Synonym(s):
GR, Glutathione-disulfide reductase, NADPH:oxidized glutathione oxidoreductase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.41

recombinant

expressed in E. coli

Quality Level

form

buffered aqueous solution

specific activity

≥10 units/mg protein

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GSR(2936)

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This Item
G3664SAB4200182T7915
form

buffered aqueous solution

form

ammonium sulfate suspension

form

buffered aqueous solution

form

ammonium sulfate suspension

specific activity

≥10 units/mg protein

specific activity

100-300 units/mg protein (biuret)

specific activity

-

specific activity

>25 units/mg protein (Bradford)

UniProt accession no.

P00390

UniProt accession no.

P41921

UniProt accession no.

P00390

UniProt accession no.

P0A9P4

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Application

Human glutathione reductase has been used in:
  • glutathione reductase activity assay
  • oxidative stress analysis
  • redox assays

Biochem/physiol Actions

Glutathione reductase enzyme is a homodimeric enzyme containing 1 FAD molecule and 1 NADPH binding domain per subunit. Both human GR (hGR) and Plasmodium falciparum GR (PfGR) are essential for the survival of the malaria parasite within the human erythrocyte. Thus, this enzyme may be used for studies of candidate anti-malaria reagents.
Glutathione reductase is a ubiquitous flavoenzyme involved in the protection from cell stress. Glutathione reductase catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). It is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also an electron donor for glutathione peroxidases and a substrate for glutathione S-transferases contributing to the detoxification and elimination of toxic electrophilic metabolites and xenobiotics.

Unit Definition

1 unit will reduce 1.0 μmole of DTNB to TNB per minute at 25 °C at pH 7.5.

Physical form

Solution containing 25 mM Tris-HCl, pH 7.4, 1 mM EDTA, and 50% (v/v) glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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