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Sigma-Aldrich

Glutathione Reductase Assay Kit

Sufficient for 100 colorimetric tests

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Quality Level

usage

sufficient for 100 tests

detection method

colorimetric

storage temp.

2-8°C

Gene Information

human ... GSR(2936)

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This Item
CS0260CS0170MAK364
Glutathione Assay Kit sufficient for 700 assays

Sigma-Aldrich

CS0260

Glutathione Assay Kit

detection method

colorimetric

detection method

-

detection method

-

detection method

colorimetric

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Gene Information

human ... GSR(2936)

Gene Information

human ... GSR(2936)

Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Gene Information

-

General description

Glutathione reductase (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. 

Application

Glutathione Reductase Assay Kit has been used to measure the activity of glutathione reductase as a part of oxidative stress assessment and also to study the effects of antifouling biocides on it.

Biochem/physiol Actions

Glutathione reductase (EC 1.6.4.2) (GR) is a ubiquitous enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also a substrate for the glutathione peroxidases and glutathione S-transferases in the detoxification of organic peroxides and the metabolism of xenobiotics. This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).
This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).

Suitability

Suitable for the measurement of glutathione reductase activity in biological samples

Principle

This kit contains reagents for the spectrophotometric determination of glutathione reductase activity either by following the decrease in absorbance caused by the oxidation of NADPH at 340 nm (UV assay) or the increase in absorption caused by the reduction of dithiobis(2-nitrobenzoic acid) (DTNB) at 412 nm (colorimetric assay). This kit provides reagents for a spectrophotometric assay for measuring the activity of glutathione reductase either by following the decrease in A340 caused by the oxidation of NADPH or the increase in A412 caused by the reduction of dithiobis(2-nitrobenzoic acid).

Analysis Note

UV assay: One unit will cause the oxidation of 1.0 μmole of NADPH at 25 °C at pH 7.5.
Colorimetric assay: One unit will cause the reduction of 1.0 μmole of DTNB to TNB at 25 °C at pH 7.5.

Kit Components Also Available Separately

Product No.
Description
SDS

  • D81305,5′-Dithiobis(2-nitrobenzoic acid) 50 mgSDS

  • G4626Glutathione, oxidized, disodium salt 100 mgSDS

  • N6505β-Nicotinamide adenine dinucleotide 25 mgSDS

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A method for assaying glutathione reductase (GSH; EC 1.6.4.2) in crude plant extracts is described. The method is based on the increase in absorbance at 412 nm when 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) is reduced by GSH. The effects of the following
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