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38185

Sigma-Aldrich

Quantification kit for oxidized and reduced glutathione

200 tests

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Synonym(s):
γ-L-glutamyl-L-cysteinylglycine kit, Quantification kit
UNSPSC Code:
12352108
NACRES:
NA.32

Quality Level

quality

200 tests

storage temp.

2-8°C

General description

Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.

Application

Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.

Features and Benefits

The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.

Components

Glutathione Quantification Kit contains:
  • Enzyme Solution (50µl X 1)
  • Buffer Solution (60ml X 1)
  • Standard GSH (X 1)
  • Masking Reagent (20µl X 1)
  • Co-enzyme (X 2)
  • Substrate (DTNB) (X4)
  • Standard GSSG (X 1)

Analysis Note

Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.

Other Notes

not available in Korea, Singapore, Taiwan and Japan

pictograms

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Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1

wgk_germany

WGK 3


Certificates of Analysis (COA)

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Anna Maria Czarnecka et al.
Nutrients, 12(7) (2020-07-28)
Acute liver failure (ALF) impairs cerebral function and induces hepatic encephalopathy (HE) due to the accumulation of neurotoxic and neuroactive substances in the brain. Cerebral oxidative stress (OS), under control of the glutathione-based defense system, contributes to the HE pathogenesis.
Hidemasa Katsumi et al.
Metallomics : integrated biometal science, 6(5), 1050-1056 (2014-03-25)
Reactive oxygen species (ROS) are involved in the pathophysiology of ischemia/reperfusion injury. To protect mouse hepatocytes from ischemia/reperfusion injury, we prepared two different sizes of citric acid-protected platinum nanoparticles (Pt-NPs), which exhibited ROS-scavenging activities and selective delivery to a specific
Hayato Nakagawa et al.
Cancer cell, 26(3), 331-343 (2014-08-19)
Endoplasmic reticulum (ER) stress has been implicated in the pathogenesis of viral hepatitis, insulin resistance, hepatosteatosis, and nonalcoholic steatohepatitis (NASH), disorders that increase risk of hepatocellular carcinoma (HCC). To determine whether and how ER stress contributes to obesity-driven hepatic tumorigenesis
Hany H Arab et al.
Chemico-biological interactions, 335, 109368-109368 (2021-01-08)
Dapagliflozin, a selective sodium-glucose co-transporter 2 (SGLT2) inhibitor, has featured marked anti-inflammatory effects in murine models of myocardial infarction, renal injury, and neuroinflammation. Yet, its potential impact on the pathogenesis of inflammatory bowel diseases (IBD) has not been previously investigated.
Roza Szatkowska et al.
The Biochemical journal, 476(7), 1053-1082 (2019-03-20)
Protein biosynthesis is energetically costly, is tightly regulated and is coupled to stress conditions including glucose deprivation. RNA polymerase III (RNAP III)-driven transcription of tDNA genes for production of tRNAs is a key element in efficient protein biosynthesis. Here we

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