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D224

Sigma-Aldrich

4,5-Diaminofluorescein

5 mM in DMSO, ≥97% (HPLC)

Synonym(s):

DAF-2

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About This Item

MDL number:
UNSPSC Code:
41116100
NACRES:
NA.32

Quality Level

assay

≥97% (HPLC)

form

solution

availability

not available in Japan

concentration

5 mM in DMSO

storage temp.

2-8°C

Application

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

Analysis Note

Final solution contains approx. 10% isopropanol by volume.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Georgina A Rivera-Ingraham et al.
Frontiers in zoology, 13, 4-4 (2016-02-05)
Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize
Julia Strahl et al.
PloS one, 15(5), e0232360-e0232360 (2020-05-08)
The free radical nitric oxide (NO) is a powerful metabolic regulator in vertebrates and invertebrates. At cellular concentrations in the nanomolar range, and simultaneously reduced internal oxygen partial pressures (pO2), NO completely inhibits cytochrome-c-oxidase (CytOx) activity and hence mitochondrial- and
Isabella Echeverri et al.
American journal of human biology : the official journal of the Human Biology Council, 27(6), 822-831 (2015-05-07)
To evaluate the Relationship between maternal and newborn endothelial function and oxidative stress. Forty-three pregnant women and their offspring were evaluated. As markers of endothelial function, the flow-mediated dilation (FMD) was measured in pregnant women in the second and third

Articles

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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