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D6442

Sigma-Aldrich

JumpStart Taq ReadyMix for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

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form

liquid

usage

sufficient for 20 reactions
sufficient for 2000 reactions
sufficient for 400 reactions

feature

dNTPs included
hotstart

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

compatibility

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

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D7440QR0200P1107
vibrant-m

QR0200

Quantitative RT-PCR ReadyMix

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

qPCR: suitable

technique(s)

qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

PCR: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

usage

sufficient for 20 reactions, sufficient for 400 reactions, sufficient for 2000 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

usage

sufficient for 250 reactions

usage

sufficient for 100 reactions, sufficient for 400 reactions

input

purified DNA

input

purified DNA

input

purified RNA

input

purified DNA

General description

JumpStart Taq ReadyMix For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.

Application

JumpStart Taq ReadyMix for High Throughput Quantitative PCR has been used:
  • For quantitative polymerase chain reaction (qPCR) of cDNA template
  • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes
  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

Features and Benefits

  • JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • When performing large number of PCR reactions, JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps
  • This master mix allows consistent batch-to-batch and reaction-to-reaction performance
  • Internal Reference Dye is provided for reaction normalization

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Quantity

Use 2.5 units per reaction.

Legal Information

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Related product

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 3


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