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R2523

Sigma-Aldrich

REDTaq® ReadyMix PCR Reaction Mix

Complete PCR reagent with standard Taq DNA Polymerase and inert dye

Synonym(s):
PCR master mix, PCR master mix with loading dye, Taq PCR master mix
NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 20 reactions

feature

dNTPs included
hotstart: no

technique(s)

PCR: suitable

color

red

input

purified DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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This Item
P4600P1107R4775
usage

sufficient for 100 reactions

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 400 reactions

usage

sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart

feature

dNTPs included, hotstart

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

-

color

red

color

colorless

color

red

color

red

input

purified DNA

input

purified DNA

input

purified DNA

input

-

General description

REDTaq® ReadyMix is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. The red loading dye migrates at approximately the same rate as a 125 base pair fragment in a 1% agarose gel. The inert dye has no effect on the amplification process, and therefore, a sample can be easily re-amplified such as in “nested PCR”.

Application

REDTaq® ReadyMix PCR Reaction Mix has been used:
  • for routine polymerase chain reaction (PCR) amplification.
  • for the PCR amplification of cDNA
  • in semi-quantitative PCR

Features and Benefits

The benefits of REDTaq® with the convenience of ReadyMix
  • Saves preparation time
  • Reduces risk of contamination from multiple pipet steps and and increased reproducibility.
  • Provides consistent reaction-to-reaction performance
  • No loading buffer/tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification
  • The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue)
  • Amplifies targets up to 7 kb in length
  • Because no loading buffer has been added, a sample can be easily re-amplifed such as in nested PCR.
  • Stable at 4°C or −20°C.
  • Ready-mix format reduces setup time.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL

Preparation Note

Add 25 μl of ReadyMix to template, primers and water to a final reaction volume of 50 μl. The PCR product is easily separated from the dye by standard purification methodologies. The inert red dye has no effect on automated sequencing and is suitable for restriction enzyme digestion, ligation and other downstream manipulations.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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