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12140314001

Roche

High Fidelity PCR Master

sufficient for ≤200 reactions, kit of 1 (2 components), suitable for PCR

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Synonym(s):
High fidelity PCR | PCR

usage

sufficient for ≤200 reactions

Quality Level

feature

High Fidelity PCR
dNTPs included
hotstart: no

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

2-8°C

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This Item
11636103001FPCR-ROEHIFIP-RO
vibrant-m

12140314001

High Fidelity PCR Master

vibrant-m

11636103001

PCR Master

vibrant-m

FPCR-RO

FastStart PCR Master

usage

sufficient for ≤200 reactions

usage

sufficient for 200 reactions, (50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase)

usage

sufficient for ≤2,000 reactions (04710452001), sufficient for ≤100 reactions (04710436001), sufficient for ≤400 reactions (04710444001)

usage

sufficient for ≤2,500 reactions (03300234001), sufficient for 2500 reactions, sufficient for ≤500 reactions (03300226001), sufficient for 500 reactions

feature

High Fidelity PCR

feature

dNTPs included, hotstart: no

feature

-

feature

High Fidelity PCR, dNTPs included: no, hotstart: no

packaging

kit of 1 (2 components)

packaging

kit of 1 (2 components)

packaging

pkg of 2 × 1.25 mL (04710436001), pkg of 8 × 1.25 mL (04710444001), pkg of 10 × 5 mL (04710452001)

packaging

pkg of 2,500 U (03300234001 [10x250 U]), pkg of 500 U (03300226001 [2x250 U])

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

input

purified DNA

input

purified DNA

input

-

input

purified DNA

General description

The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→ 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
PCR products have both blunt ends and 3′ single A-overhangs just like using Taq DNA polymerase, ideal for blunt end cloning or T/A cloning.
The High Fidelity PCR Master is a ready-to-use, double-concentrated master mix that contains all the reagents required to perform PCR (except template and primer). Water, PCR Grade, is also supplied.
This powerful specific enzyme blend of the High Fidelity PCR Master is designed to give PCR products with high yield, high fidelity, and high specificity from episomal and genomic DNA.
The special enzyme blend is composed of two thermostable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. Due to the inherent 3′-5′ exonuclease activity of the proofreading polymerase, the High Fidelity PCR Master results in approximately three times the fidelity and approximately twice the yield of Taq DNA Polymerase alone.

Application

  • Amplify longer fragments with more fidelity
up to 5 kb.
  • Reduce setup time.
Store at +2 to +8ºC – ready for immediate use – without thawing.
  • Minimize contamination with two pipetting steps:
Add primers and template.
Choose the convenient High Fidelity PCR Master, a double-concentrated, ready-to-use mix, which combines Expand High Fidelity PCR System with PCR-Grade dNTPs, MgCl2, and an optimized reaction buffer. The High Fidelity PCR Master delivers twice the yield and three times better fidelity than Taq DNA Polymerase alone.High yield and increased fidelity compared to Taq DNA Polymerase. Use the High Fidelity PCR Master for:

  • PCR up to 5 kb
  • RT-PCR up to 5 kb

High Fidelity PCR Master is stable at +2 to +8°C, allowing PCR without time-consuming reagent thawing.

Packaging

1 kit containing 2 components.

Quality

Each lot is PCR function tested using human genomic DNA and primers specific for a 4.8 kb fragment of the tPA gene.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit Components Only

Product No.
Description

  • High Fidelity PCR Master mix containing enzyme blend, reaction buffer, and nucleotides (10 vials)

  • Water, PCR Grade (5 vials)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Articles

A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.

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