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JumpStart REDTaq® ReadyMix Reaction Mix

for PCR

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Hot start PCR master mix, Hot start master mix, Hot start taq master mix
MDL number:

Quality Level




sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 800 reactions


dNTPs included


2.5 units/reaction (50 μL reaction volume)


PCR: suitable




purified DNA



shipped in

wet ice

storage temp.


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This Item

sufficient for 100 reactions


sufficient for 100 reactions, sufficient for 20 reactions


sufficient for 100 reactions, sufficient for 400 reactions


sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions


dNTPs included


dNTPs included, hotstart: no


Multiplex PCR, dNTPs included, hotstart


dNTPs included, hotstart


2.5 units/reaction (50 μL reaction volume)




2.5 units/reaction (50 μL reaction volume)


1.25 units/reaction (50 μL reaction volume)


PCR: suitable


PCR: suitable


PCR: suitable


qPCR: suitable









General description

JumpStart REDTaq® ReadyMixPCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.


Jumpstart REDTaq® ReadyMix Reaction Mix is used for direct DNA extraction and amplification using PCR. It has also been used for:
  • qualitative multiplex PCR
  • methylation-specific PCR (MSPCR)
  • PCR amplification of cDNA synthesized from total RNA

Features and Benefits

  • JumpStart REDTaq® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
  • REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
  • Inert red dye allows for easy verification that reagent has been mixed properly
  • Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction


Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and ReadyMix products at
For a typical PCR reaction, mix 25 μL of JumpStart REDTaq® ReadyMix with 25 μL of a mixture containing template DNA, primers, and water. Reaction volume can be scaled if desired.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Assaad Semaan et al.
Pharmaceutical research, 28(12), 3079-3090 (2011-08-06)
MicroRNA-101 (miR-101) expression is negatively associated with tumor growth and proliferation in several solid epithelial cancers. Enhancer of zeste homolog 2 (EzH2) appears to be a functional target of miR-101. We explore the role of miR-101 and its interaction with
Tatiana V Danilova et al.
Chromosoma, 121(6), 597-611 (2012-10-12)
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA
Wendy Monger et al.
Archives of virology, 163(6), 1585-1594 (2018-03-02)
A novel virus was discovered in a freeze-dried collection held at SASA, UK, originating from potato (Solanum tuberosum) cv. Nadine. The complete sequence of the viral RNA was determined to be 3674 nucleotides in length encoding five predicted proteins. Based
A DNA methylation microarray-based study identifies ERG as a gene commonly methylated in prostate cancer
Schwartzman J, et al.
Epigenetics, 6, 1248-1256 (2011)
The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
Snapkov I, et al.
BMC Cancer, 16, 490-490 (2016)


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