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P1107

Sigma-Aldrich

JumpStart REDTaq® ReadyMix Reaction Mix

for High-throughput PCR of complex templates

Synonym(s):

High throughput PCR, Hot start PCR master mix, Hot start master mix, Hot start taq master mix

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About This Item

MDL number:
UNSPSC Code:
41106300
NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 100 reactions
sufficient for 400 reactions

feature

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

concentration

0.75 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

red

input

purified DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
JumpStart REDTaq® ReadyMixPCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

Application

JumpStartREDTaq®ReadyMix Reaction Mix has been used:
  • For multiplex PCR
  • For amplification with reduced non-specific amplification
  • For high throughput PCR amplification of complex templates
  • In the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis
  • For PCR amplification of the human TMPRSS6 gene

Features and Benefits

  • JumpStart REDTaq® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
  • REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
  • Inert red dye allows for easy verification that reagent has been mixed properly
  • Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Beutler et al.
Blood cells, molecules & diseases, 44(1), 16-21 (2009-10-13)
Male subjects with iron deficiency from the general population were examined for polymorphisms or sporadic mutations in TMPRSS6 to identify genetic risk factors for iron deficiency anemia. Three uncommon non-synonymous polymorphisms were identified, G228D, R446W, and V795I (allele frequencies 0.0074
Jarred Yasuhara-Bell et al.
International journal of systematic and evolutionary microbiology, 65(Pt 3), 811-826 (2014-12-08)
The genus Clavibacter contains one recognized species, Clavibacter michiganensis. Clavibacter michiganensis is subdivided into subspecies based on host specificity and bacteriological characteristics, with Clavibacter michiganensis subsp. michiganensis causing bacterial canker of tomato. Clavibacter michiganensis subsp. michiganensis is often spread through
John C Hafner et al.
Journal of biogeography, 38(6), 1077-1097 (2011-08-13)
AIM: The rodent genus Microdipodops (kangaroo mice) includes two sand-obligate endemics of the Great Basin Desert: M. megacephalus and M. pallidus. The dark kangaroo mouse, M. megacephalus, is distributed throughout the Great Basin and our principal aims were to formulate
Richard Splivallo et al.
Frontiers in microbiology, 10, 1437-1437 (2019-07-19)
Truffle fungi are well known for their enticing aromas partially emitted by microbes colonizing truffle fruiting bodies. The identity and diversity of these microbes remain poorly investigated, because few studies have determined truffle-associated bacterial communities while considering only a small
Wenqin Wang et al.
BMC plant biology, 10, 205-205 (2010-09-18)
Members of the aquatic monocot family Lemnaceae (commonly called duckweeds) represent the smallest and fastest growing flowering plants. Their highly reduced morphology and infrequent flowering result in a dearth of characters for distinguishing between the nearly 38 species that exhibit

Articles

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Protocols

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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