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D9443

SAFC

Dulbecco′s Modified Eagle′s Medium - low glucose

liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications

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Synonym(s):
DME, DMEM
MDL number:
NACRES:
NA.75

description

With 1000mg/L D-glucose, L-glutamine, and sodium bicarbonate. Without arginine, leucine, lysine, sodium pyruvate, and phenol red

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
D6171D0422D1152
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

components

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes

components

glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

500

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mahesh Saqcena et al.
PloS one, 8(8), e74157-e74157 (2013-08-27)
In multicellular organisms, cell division is regulated by growth factors (GFs). In the absence of GFs, cells exit the cell cycle at a site in G1 referred to as the restriction point (R) and enter a state of quiescence known
A M Edick et al.
Journal of dairy science, 104(1), 1123-1135 (2020-11-10)
The objective of this study was to determine the role of GCN2 in the response to AA deprivation of primary bovine mammary epithelial cells (BMEC). Cells were isolated from the mammary tissue of 2 lactating Holstein cows by enzymatic digestion
Dipsikha Biswas et al.
Cell death discovery, 7(1), 241-241 (2021-09-17)
Triple-negative breast cancers (TNBCs) are characterized by poor survival, prognosis, and gradual resistance to cytotoxic chemotherapeutics, like doxorubicin (DOX). The clinical utility of DOX is limited by its cardiotoxic and chemoresistant effects that manifest over time. To induce chemoresistance, TNBC
Sona Hubackova et al.
Molecular oncology, 13(7), 1467-1489 (2019-03-29)
Radiation and chemotherapy represent standard-of-care cancer treatments. However, most patients eventually experience tumour recurrence, treatment failure and metastatic dissemination with fatal consequences. To elucidate the molecular mechanisms of resistance to radio- and chemotherapy, we exposed human cancer cell lines (HeLa
Dipsikha Biswas et al.
The Journal of biological chemistry, 295(46), 15597-15621 (2020-09-04)
Branched-chain α-keto acids (BCKAs) are catabolites of branched-chain amino acids (BCAAs). Intracellular BCKAs are cleared by branched-chain ketoacid dehydrogenase (BCKDH), which is sensitive to inhibitory phosphorylation by BCKD kinase (BCKDK). Accumulation of BCKAs is an indicator of defective BCAA catabolism

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