E1257

Epidermal Growth Factor from murine submaxillary gland

lyophilized powder, BioReagent, suitable for cell culture

• Synonym(s): EGF, Epidermal cell growth factor
• CAS Number: 62229-50-9
• EC Number: 263-468-7
• MDL number: MFCD01634807
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse submaxillary glands
• Quality Level: 200
• product line: BioReagent
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.02-2.0 ng/mL EC₅₀
• packaging: pkg of 0.1 mg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• UniProt accession no.: P01132
• storage temp.: 2-8°C
• Gene Information: mouse ... Egf(13645)

Description

General description

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.

Application

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats
• for preparation of MCF-10A cell line
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)

Biochem/physiol Actions

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.

Physical form

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Preparation Note

Purified by gel filtration and ion-exchange chromatography.

Analysis Note

The biological activity is measured in a proliferation assay using BALB/MK cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)