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E2520

Sigma-Aldrich

Anti-Epidermal Growth Factor antibody, Mouse monoclonal

clone EGF-10, purified from hybridoma cell culture

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Synonym(s):
Anti-CEGF
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

EGF-10, monoclonal

form

buffered aqueous solution

mol wt

antigen 6 kDa

species reactivity

human

technique(s)

dot blot: 0.1 μg/mL using recombinant human EGF
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue
indirect ELISA: suitable
microarray: suitable
neutralization: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGF(1950)

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This Item
E3138V9134V6389
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

clone

EGF-10, monoclonal

clone

F4, monoclonal

clone

KDR-1, monoclonal

clone

V9, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

Specificity

Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

Immunogen

recombinant human EGF

Application

Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.
Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Effects of triple treatment with octreotide, galanin and serotonin on a human pancreas cancer cell line in xenografts
El-Salhy M et al.
Histology and Histopathology, 14(6), 3550-3558 (2005)
Epidermal growth factor (EGF) in the human placental infection with Trypanosoma cruzi
Lin S, et al.
Placenta, 25(4), 283-286 (2004)
Marie McIlroy et al.
Cardiovascular research, 69(1), 207-217 (2005-09-17)
Pericytes, located in close proximity to the underlying endothelium, form an integral component of the microvasculature. These cells are intimately involved in angiogenesis, which is of fundamental importance in many physiological and pathological processes. We evaluated the influence of pericyte-conditioned
Clémence Dubois et al.
Journal of clinical medicine, 8(3) (2019-03-25)
Triple-negative breast cancers are particularly aggressive. In vitro cultures are one of the major pathways for developing anticancer strategies. The effectiveness and reproducibility of the drug screenings depend largely on the homogeneity of culture media. In order to optimize the
ErbB3 is involved in activation of phosphatidylinositol 3-kinase by epidermal growth factor.
Soltoff S P, et al.
Molecular and Cellular Biology, 14(6), 3550-3558 (1994)

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