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E1157

Sigma-Aldrich

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-EGFR, Anti-ERBB, Anti-HER1, Anti-PIG61
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

capture ELISA: 0.4 μg/mL
flow cytometry: 25 μg/mL
immunoprecipitation (IP): 1 μg/mL
western blot: 1-2 μg/mL

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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This Item
E0657AMAB90819SAB5700347
biological source

goat

biological source

mouse

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

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Immunogen

purified, NSO cell-derived recombinant human epidermal growth factor receptor extracellular domain.

Application

Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat was used for immunoblotting to analyze stable expression of anti-EGFR shRNA in MCF10A breast epithelial cell line.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Fatimah Matalkah et al.
Breast cancer research : BCR, 18(1), 2-2 (2016-01-06)
Dysregulated receptor tyrosine kinase (RTK) signaling is a common occurrence in basal-like and triple-negative breast cancer (BTBC). As a result, RTK-targeting therapies have been initiated but proved difficult, mainly owing to the multiplicity of dysregulated RTKs. Hence, targeting master regulators
Xiangdong Zhou et al.
Cellular signalling, 24(1), 140-150 (2011-09-14)
Human epidermal growth factor receptor 2 (HER2) belongs to the EGFR family of receptor tyrosine kinases that comprises four members. As opposed to the other family members, HER2 does not require ligand binding for activation. Hence, HER2 molecules can undergo
Z Hartman et al.
Oncogene, 32(35), 4169-4180 (2012-10-03)
One of the causes of breast cancer is overexpression of the human epidermal growth factor receptor 2 (HER2). Enhanced receptor autophosphorylation and resistance to activation-induced downregulation have been suggested as mechanisms for HER2-induced sustained signaling and cell transformation. However, the
Thu H Truong et al.
Biochemistry, 51(50), 9954-9965 (2012-11-29)
Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor
Jing Bai et al.
Asian Pacific journal of cancer prevention : APJCP, 13(10), 4879-4881 (2012-12-19)
Epidermal growth factor receptor (EGFR) overexpression is associated with resistance to chemotherapy and radiotherapy. The EGFR modulates DNA repair after radiation-induced damage through an association with the catalytic subunit of DNA protein kinase. DNA double-strand breaks (DSBs) are the most

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