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E2760

Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 29.1, ascites fluid

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Synonym(s):
Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

29.1, monoclonal

contains

15 mM sodium azide

species reactivity

pig, human, rat, mouse, canine

should not react with

rabbit

technique(s)

indirect ELISA: 1:10,000 using extract of the A431 cell line
western blot: 1:3,000 using extract of the A431 cell line

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

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This Item
E3138E2156E2520
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

29.1, monoclonal

clone

F4, monoclonal

clone

225, monoclonal

clone

EGF-10, monoclonal

species reactivity

pig, human, rat, mouse, canine

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

indirect ELISA: 1:10,000 using extract of the A431 cell line, western blot: 1:3,000 using extract of the A431 cell line

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:2,000

technique(s)

immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells

technique(s)

dot blot: 0.1 μg/mL using recombinant human EGF, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue, indirect ELISA: suitable, microarray: suitable, neutralization: suitable, radioimmunoassay: suitable, western blot: suitable

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General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R).

Specificity

Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.

Immunogen

human carcinoma cell line A431, which expresses high levels of EGF receptors.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunoaffinity
  • immunohistochemistry
  • western blotting

Biochem/physiol Actions

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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B Assarsson et al.
Acta physiologica Scandinavica, 154(2), 177-183 (1995-06-01)
Members of the epidermal growth factor (EGF) family have many different effects on ovarian cells including a strong inhibition of estradiol-17 beta production. An increased binding of the EGF family to its receptor is seen at the time of ovulation.
Hong Suk Song et al.
Cancer research and treatment : official journal of Korean Cancer Association, 36(4), 240-245 (2004-08-01)
The purpose of this study was to investigate the prognostic significance of the expression of EGFR and C-erbB-2 gene products by immunohistochemical analysis for curatively resected gastric adenocarcinoma. Between January 1996 and December 2001, 739 patients with curatively resected gastric
Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer
Mitsudomi T and Yatabe Y
The FEBS journal, 277(2), 301-308 (2010)
Nicole Salazar et al.
Molecular cancer, 13, 198-198 (2014-08-30)
Recent advances have revealed a significant contribution of chemokines and their receptors in tumor growth, survival after chemotherapy, and organ-specific metastasis. The CXC chemokine receptor-7 (CXCR7) is the latest chemokine receptor implicated in cancer. Although over expressed in breast cancer
Nanoscale imaging of epidermal growth factor receptor clustering effects of inhibitors
Abulrob A, et al.
The Journal of biological chemistry, 285(5), 3145-3156 (2010)

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