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E2645

Sigma-Aldrich

Epidermal Growth Factor Receptor human

lyophilized powder, ≥15,000 units/mg protein (Bradford)

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Synonym(s):
ERBB, ErbB1, HER1, EGFR
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

form

lyophilized powder

potency

>15000.00 units/mg

specific activity

≥15,000 units/mg protein (Bradford)

mol wt

~170 kDa

packaging

pkg of 500UN

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell based assay: suitable

color

white

solubility

glycerol: 10%, clear to slightly hazy, colorless

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EGFR(1956)

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This Item
E5036E3641E1257
Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P00533

UniProt accession no.

P01133

UniProt accession no.

P00533

UniProt accession no.

P01132

packaging

pkg of 500UN

packaging

pkg of 200 and 500 μg

packaging

pkg of 500UN

packaging

pkg of 0.1 mg

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

General description

Research area: Cell signaling

Epidermal growth factor receptor (EGFR) belongs to a family of cell surface receptors called receptor tyrosine kinases (RTKs) and is also the most studied member of this family. The EGFR is encoded by the ErbB gene, mapped to chromosome 7 q22. These receptors are attached to the cytoplasmic membrane and consist of an ectodomain (ECD), comprising of four subdomains called DI-DIV, a hydrophobic transmembrane domain, and an intracytoplasmic tyrosine kinase domain.

Biochem/physiol Actions

EGF exerts its actions by binding to the EGF receptor (EGFR), a 170 kDa glycoprotein having EGF-activated protein tyrosine kinase activity. Even in the absence of EGF-like ligands, EGFR can participate in cellular responses elicited by several other stimuli. High levels of EGFR are expressed in approximately one third of human epithelial tumors, and in the cancers of the bladder, breast or lung that have poor clinical prognosis, hence the interest in targeting EGFR.
Epidermal growth factor receptor (EGFR) has an essential role in normal developmental processes, and is mutated in multiple cancers. It is involved in the pathogenesis of epidermoid tumors, and thus, has extreme importance as a cancer therapeutic target. Gefitinib (GEF) and erlotinib act as EGFR inhibitors, and induce apoptosis in non-small cell lung cancer (NSCLC) cells. A truncated isoform, called EGFRvIII is linked to multiple cases of head and neck squamous cell carcinoma (HNSCC). In ∼40% of glioblastoma multiforme (GBM), this gene is amplified.

Unit Definition

One unit (U) of the enzyme is defined as the amount needed to incorporate 1 pmol of phosphate into the substrate (KVEKIGEGTYGVVYK: 6 - 20 residue of p34cdc2) in 1 minute.

Physical form

Lyophilized from a solution in 50 mM HEPES, pH 7.6, 150 mM NaCl, 0.05% Triton X-100, 1 mM dithiothreitol, and 10% trehalose.

Preparation Note

Affinity purified from human carcinoma A431 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Qian Gou et al.
Oncogene, 39(25), 4844-4853 (2020-05-23)
In response to nutrient deficiency, autophagy degrades cytoplasmic materials and organelles in lysosomes, which is nutrient recycling, whereas activation of EGFR mediates autophagy suppression in response to growth factors. It is unclear whether PPARδ could be the regulator of autophagy
EGFR/MDM2 signaling promotes NF-?B activation via PPAR? degradation
Xu Y, et al.
Carcinogenesis, 37(2), 215-222 (2016)
Epidermal Growth Factor Receptor Cell Proliferation Signaling Pathways
Wee P and Wang Z
Cancers, 9(5), 52-52 (2017)
A comprehensive pathway map of epidermal growth factor receptor signaling
Oda K, et al.
Molecular Systems Biology (2005)
The Epidermal Growth Factor Receptor Pathway: A Model for Targeted Therapy
Scaltriti M and Baselga J
Journal of Separation Science null

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