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E3138

Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone F4, ascites fluid

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Synonym(s):
Anti-EGFR
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

contains

15 mM sodium azide

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:2,000

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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This Item
E2760E2156SAB4200809
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

F4, monoclonal

clone

29.1, monoclonal

clone

225, monoclonal

clone

29.1, monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

species reactivity

human

species reactivity

pig, human, rat, mouse, canine

species reactivity

human

species reactivity

human, bovine, mouse

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General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.

Specificity

This antibody recognizes specifically an intracellular domain of the EGF receptor.

Immunogen

synthetically produced residues 985-996 of the human EGF receptor.

Application

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemical staining

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Journal of Molecular Histology, 38(3), 207-214 (2007)
Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Armstrong J and Childs GV
Endocrinology, 138(5), 1903-1908 (1997)
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway
Steen H, et al.
The Journal of Biological Chemistry, 277(2), 1031-1039 (2002)
Yuen-Keng Ng et al.
Melanoma research, 24(3), 207-218 (2014-04-09)
The BRAF inhibitor vemurafenib is currently used for treating patients with BRAF V600E mutant melanoma. However, the responses to vemurafenib are generally partial and of relatively short duration. Recent evidence suggests that activation of the epidermal growth factor receptor (EGFR)/erbB
T Cerny et al.
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48

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