Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

View All Documentation

E4011

Sigma-Aldrich

ExtrAvidin®−R-Phycoerythrin

buffered aqueous solution

Synonym(s):

R-Phycoerythrin

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

MDL number:
MFCD00163600
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

phycoerythrin (R-PE) conjugate

Quality Level

200

form

buffered aqueous solution

storage condition

protect from light

extent of labeling

1-4 mol PE per mol ExtrAvidin®

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

storage temp.

2-8°C

General description

ExtrAvidin is a chemically modified protein purified from egg whites.
The extravidin-R-phycoerythrin protein can be conjugated with fluorescein-labelled arylacetamide, a κ opioid ligand and biotin-conjugated anti-fluorescein IgG to identify the subpopulations of thymocytes that express κ opioid receptor. This product has shown specificity for rabbit anti-avidin in immunoelectrophoresis analysis.
ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Immunogen

Thymocytes present in C57BL mice.

Application

ExtrAvidin®-R-Phycoerythrin has been used
  • In flow cytometric analysis to confirm membrane expression of ncamp-1 (cationic oligodeoxynucleotide binding protein).
  • In indirect immunofluorescence.
  • In Western blotting.

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

C-Phycocyanin from Spirulina sp. lyophilized powder

Sigma-Aldrich

P2172

C-Phycocyanin from Spirulina sp.

D M Lawrence et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(4), 1062-1066 (1995-02-14)
A method to visualize the kappa opioid receptor is described that uses a high-affinity fluorescein-conjugated opioid ligand and indirect immunofluorescence with the phycoerythrin fluorophore to amplify the signal. The mouse thymoma cell line R1E/TL8x.1.G1.OUAr.1 (R1EGO), which expresses the kappa 1
T A Ignatowski et al.
The Journal of pharmacology and experimental therapeutics, 284(1), 298-306 (1998-01-22)
Recent studies have shown kappa opioid receptor labeling on the R1EGO thymoma cell line by indirect immunofluorescence and flow cytometric analysis. The present study used a fluorescein-labeled arylacetamide (FITC-AA), a kappa opioid ligand, in conjunction with biotin-conjugated anti-fluorescein IgG and
Detection of opioid receptors on murine lymphocytes by indirect immunofluorescence: mature normal and tumor bearing mice lymphocytes
Karaji AG, et al.
International Immunopharmacology, 5(6), 1019-1027 (2005)
In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor
Schimanski LM, et al.
Blood Cells, Molecules and Diseases, 43(2), 180-193 (2009)
Donald L Evans et al.
Developmental and comparative immunology, 29(12), 1049-1064 (2005-07-07)
Nonspecific cytotoxic cells (NCC) are the first identified and most extensively studied killer cell population in teleosts. NCC kill a wide variety of target cells including tumor cells, virally transformed cells and protozoan parasites. The present study identified a novel
View All Related Papers

Articles

Flow Cytometry Dye Selection Tips

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Flow Cytometry Troubleshooting Guide

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

Protocols

Key Steps in Flow Cytometry Protocols

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Flow Cytometry Guide

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service