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F5392

Sigma-Aldrich

Fibroblast Growth Factor-Basic from bovine pituitary

FGF-Basic, suitable for cell culture

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Synonym(s):
FGF2, bFGF
CAS Number:
MDL number:
NACRES:
NA.77

biological source

bovine pituitary glands

Quality Level

assay

~90% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.03-3.0 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

16.4 kDa

packaging

pkg of 1 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.5 EU/vial

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

bovine ... FGF2(281161)

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This Item
F5542T5300P8953
biological source

bovine pituitary glands

biological source

human

biological source

Porcine (Platelets)

biological source

Porcine

assay

~90% (SDS-PAGE and N-terminal analysis)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥97% (SDS-PAGE and N-terminal analysis)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

packaging

pkg of 1 μg

packaging

pkg of 25 μg

packaging

pkg of 1 μg

packaging

pkg of 5 μg

General description

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:
  • In animal and human cell culture
  • In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
  • In the generation of directly reprogramming fibroblast
  • In human glioma cell culture

Biochem/physiol Actions

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Preparation Note

Purified glands by a modification of the method of Gospodarowicz, et al.

Analysis Note

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Stimulatory effects of fibroblast growth factor 2 on proliferation and migration of uterine luminal epithelial cells during early pregnancy
Lim W, et al.
Biology of Reproduction, 96(1), 185-198 (2016)
Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls
Salehi A, et al.
Molecular Biology Research Communications, 4(1), 57-57 (2015)
Kathryn A Jacobs et al.
Methods in molecular biology (Clifton, N.J.), 1749, 51-58 (2018-03-12)
Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this
Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs
Capkin M, et al.
Biomedical Materials (Bristol, England), 7(4), 45013-45013 (2012)
Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell lines stored in the National Cell Bank of Iran
Kazemiha VM, et al.
Cytotechnology, 66(5), 861-873 (2014)

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