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G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

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Synonym(s):
Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

composition

Protein, 35-65% modified Warburg-Christian

impurities

≤10 % protein α-lactalbumin

storage temp.

−20°C

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General description

Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1
  • in β -1,4-galactosyltransferase assay rice root cell extracts
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states.

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Unit Definition

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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