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G5518

Sigma-Aldrich

Anti-Goat IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:50,000
quantitative precipitin assay: 3.5 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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1 of 4

This Item
R3756R5381I1011
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

goat

technique(s)

indirect ELISA: 1:50,000, quantitative precipitin assay: 3.5 mg/mL

technique(s)

indirect ELISA: 1:90,000

technique(s)

indirect ELISA: 1:100,000, quantitative precipitin assay: 2.8 mg/mL

technique(s)

indirect ELISA: 1:40,000, quantitative precipitin assay: 4.0 mg/mL

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

whole antiserum

antibody form

whole antiserum

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

IgG is a glycoprotein. It is a major class of immunoglobulin. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Immunogen

Anti-Goat IgG is produced in rabbit using as immunogen purified goat IgG.

Application

Anti-Goat IgG (whole molecule) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) assay and electron microscopy.
Anti-Goat IgG (whole molecule) antibody produced in rabbit was used in combination with protein A-colloidal gold conjugate in electron microscopy of in vitro matured and fertilized boar oocytes.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)

Biochem/physiol Actions

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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The primate-specific microRNA gene cluster (C19MC) is imprinted in the placenta
Noguer-DM, et al.
Human Molecular Genetics, 19(18), 3566-3582 (2010)
Different BCR/Abl protein suppression patterns as a converging trait of chronic myeloid leukemia cell adaptation to energy restriction
Bono S, et al.
Testing, 7(51), 84810-84810 (2016)
Pilar Coy et al.
Human reproduction (Oxford, England), 27(7), 1985-1993 (2012-05-05)
The role of the plasminogen-plasmin (PLG-PLA) system in fertilization is unknown, although its dysfunction has been associated with subfertility in humans. We have recently detected and quantified plasminogen in the oviductal fluid of two mammals and showed a reduction in
Stefania Cannito et al.
Oncotarget, 6(4), 2206-2221 (2014-12-30)
SERPINB3 is a cysteine-proteases inhibitor up-regulated in a significant number of cirrhotic patients carrying hepatocellular carcinoma (HCC) and recently proposed as a prognostic marker for HCC early recurrence. SERPINB3 has been reported to stimulate proliferation, inhibit apoptosis and, similar to
Victor Crivianu-Gaita et al.
Biochemistry and biophysics reports, 2, 23-28 (2015-04-25)
Antibodies have widespread applications in areas ranging from therapeutics to chromatography and protein microarrays. Certain applications require only the fragment antigen-binding (Fab) units of the protein. This study compares the cleavage efficacy of dithiothreitol (DTT), mercaptoethylamine (MEA), and dithiobutylamine (DTBA)

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