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nProtein A Sepharose 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

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native protein A (S. aureus)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 5 mL


Cytiva 17-5280-01

storage condition

(20% Ehtanol)


4% cross-linked agarose

average diameter

90 μm (d50v)


2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range



>30 mg binding capacity(human IgG/ml)


suitable for bioprocess medium

storage temp.


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General description

nProtein A Sepharose 4 Fast Flow is native protein A coupled to Sepharose 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Replaces Protein A Sepharose 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

Analysis Note

To view the Certificate of Analysis for this product, please visit

Legal Information

Sepharose is a trademark of Cytiva






Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

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Eunice Cho et al.
Cell reports, 34(13), 108928-108928 (2021-04-01)
Flux through the RAF-MEK-ERK protein kinase cascade is shaped by phosphatases acting on the core components of the pathway. Despite being an established drug target and a hub for crosstalk regulation, little is known about dephosphorylation of MEK, the central
Sylwia Jones et al.
Scientific reports, 10(1), 663-663 (2020-01-22)
Antibody combinations targeting cell surface receptors are a new modality of cancer therapy. The trafficking and signalling mechanisms regulated by such therapeutics are not fully understood but could underlie differential tumour responses. We explored EGFR trafficking upon treatment with the


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